144 Carbonylated proteins and Ozone in atopic eczema

The effect of pollutants on atopic eczema (AE) has been controversial; with different proposed inflammatory mechanisms in skin such as the aryl hydrocarbon (AhR), JAK/STAT pathways, oxidative stress and Carbonylated proteins (CPs). It shown that CP levels are higher dry AE lesions. Tower Hamlets Eczema Assessment (THEA) is a clinical environmental study Bangladeshi patients. Ambient exposure THEA patients to commonly studied showed long-term ground-level Ozone (O3) strongest association severity. We aimed evaluate expression level by western blotting tape strips controls correlate it Filaggrin (FLG) genotype status. 49 10 normal ancestry were recruited. Buccal swabs taken for DNA extraction 9 tapes harvested from non-lesional site protein extraction. There was significant FLG mutations compared wild type controls/patients (Kruskal Wallis test p=0.03242). number correlated positively significantly (rho (ρ)=0.34, p=0.0095). This suggests carrying more than one mutation might be associated inducible stimuli O3 To low keratinocytes; an chamber developed. Sub-confluent immortalized human keratinocytes (NTRT) exposed 70-80 ppb 1 hour; 5 days/week over period 3 weeks. Cells split, regrown day 7 each week. Control cells pure air identical chamber, under conditions. Preliminary results 6 repeats increased activation phospho-STAT3/Total STAT3 AhR starting 2nd week O3. chronic very concentrations may AE.